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Probing the Double Bond and Phase Properties of Natural Lipid Dispersions by Cross Polarization/Magic Angle Spinning 13 C NMR
Author(s) -
Chi LangMing,
Hsieh ChemgHuain,
Wu WenGuey
Publication year - 1992
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.199200005
Subject(s) - chemistry , phosphatidylcholine , sphingomyelin , magic angle spinning , analytical chemistry (journal) , lipid bilayer phase behavior , solid state nuclear magnetic resonance , nuclear magnetic resonance spectroscopy , crystallography , lipid bilayer , phospholipid , chromatography , stereochemistry , membrane , nuclear magnetic resonance , biochemistry , physics
Solid‐state high resolution 13 C NMR spectra of bovine brain sulfatide, egg sphingomyelin, egg phosphatidylcholine, and cholesterol/phosphatidylcholine dispersions were obtained by the cross‐polarization/magic angle spinning (CP/MAS) method as functions of contact time, temperature and composition. Quantitative analysis of the chemical shift and linewidth data indicate that in the liquid‐crystalline state the hydrocarbon chains of sulfatide are packed less orderly than those of sphingomyelin, and that there are two or more conformers for the ceramide residues of natural sphingolipids. The fatty acid double bond showed higher mobility than that of sphingosine as monitored by the signal intensity profiles as a function of contact time. The same approach was used to study the coexisting Lα (liquid‐crystalline phase) and β (cholesterol‐rich characterized phase) two‐phase region of cholesterol/phosphatidylcholine mixtures. Without isotope enrichment, structural and phase properties of natural lipid dispersions can be obtained easily by monitoring the chemical shift, the signal intensity and the linewidth of l3 C NMR spectra.