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New Approaches to Absorption Detection in Capillary Chromatography and Capillary Electrophoresis
Author(s) -
Yeung Edward S.
Publication year - 1991
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.199100053
Subject(s) - chemistry , analyte , capillary electrophoresis , fluorophore , fluorescence , capillary action , absorption (acoustics) , chromatography , analytical chemistry (journal) , laser , laser induced fluorescence , optics , physics , materials science , composite material
Two new approaches for absorption detection in capillary columns are presented. They are applicable to columns as narrow as 10μm i.d. Both provide good sensitivity despite the very short optical pathlengths that are commonly available in commercial systems. The first involves irradiating the column along its axis rather than across the axis. The entire analyte zone can then participate in absorption. Gains up to 1000 fold can be expected. The second is a modification of laser‐excited fluorescence. The absorbing analytes, even though they do not fluoresce efficiently, can transfer their energy to a fluorophore added to the mobile phase. The secondary emission can be used to detect absorption in the nanomolar range.