Premium
Rapid Protein Hydrolysis by Microwave Irradiation Using Heat‐Resistant Teflon‐Pyrex Tubes
Author(s) -
Chiou ShyhHorng
Publication year - 1989
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.198900060
Subject(s) - chemistry , hydrolysis , amino acid , methanesulfonic acid , hydrolysate , peptide , chromatography , peptide bond , cystine , microwave irradiation , acid hydrolysis , organic chemistry , cysteine , biochemistry , enzyme , catalysis
A rapid peptide‐bond hydrolysis by means of microwave irradiation is introduced for the facile preparation of protein hydrolysates used for amino acid analysis. The optimal hydrolysis condition has been determined using several enzymes with known amino acid compositions. The effects of hydrolysis time on the recovery of various labile and hydrophobic amino acids are also exemplified in the microwave heating of standard amino acids. The method has been applied to the complete amino acid analysis with a single nonvolatile solvent of methanesulfonic acid with good recovery of tryptophan and half‐cystine. It provides a radical expedition of protein and peptide hydrolysis via commercial microwave ovens and specially‐designed Teflon‐Pyrex tubes, circumventing the tedious procedures using vacuum‐sealed pyrex lubes heating at 110°C for more than 24 h. This novel type of microwave chemistry associated with rapid peptide‐bond cleavage is of great potential in the automation of the complete process of amino acid analysis starting from the preparation of protein hydrolysates.