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Carbon‐13 Nuclear Magnetic Resonance Spectroscopy Study of the Glucose Stress of Human Lenses
Author(s) -
Camli Ugur,
Murray T.,
Aguayo J.,
Tarantino P.,
Hay A.,
Stark W.,
Kan LouSing
Publication year - 1989
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.198900046
Subject(s) - sorbitol , chemistry , nuclear magnetic resonance spectroscopy , spectroscopy , carbohydrate metabolism , nuclear magnetic resonance , glycolysis , carbohydrate , metabolism , biochemistry , stereochemistry , physics , quantum mechanics
Abstract The sorbitol theory in diabetic cataractogenesis was based on sorbitol accumulation under glucose stress. Sorbitol accumulation was examined by 13 C nuclear magnetic resonance spectroscopy (NMR) for the first time in matched human lenses incubated in 5.5 mM and 35.5 mM C‐1 13 C‐enriched glucose up to 28 hours. The results showed that sorbitol and lactate in human tens can be detected at 35.5 mM, but not in 5.5 mM glucose solution. The glycolysis metabolic pathway of human lenses may be quite different from that of animals. The accumulation of metabolites can be traced and quantified by the intensities of 13 C NMR peaks. Therefore, 13 C NMR spectroscopy can be used as a valuable tool to investigate human lens carbohydrate metabolism non‐interventionally.