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Reactions of Copper‐Free Hemocyanin from Taiwan Snails with Metal Salts
Author(s) -
Wang S. M.,
Chen M. N.,
Tsay J. M.,
Chung C. S.,
Li N. C.
Publication year - 1985
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.198500054
Subject(s) - hemocyanin , chemistry , cyanide , copper , absorbance , fluoride , inorganic chemistry , metal , cadmium , nuclear chemistry , organic chemistry , chromatography , genetics , antigen , biology
Copper‐free hemocyanin (apohemocyanin, apoHc) from Taiwan snails was prepared by cyanide treatment and the reconstitution of hemocyanin was carried out by treating apoHc with Cu(CH 3 CN) 4 ClO 4 . At p H below 5, the apoHc solution is cloudy. When reconstituted at p H near 6, the ratio of absorbances, A 345 / a 280 , has the normal value 0.21, and rapidly decreases with increase in p H. This indicates that reconstitution is best carried out near p H 6. Addition of fluoride at p H 5.3 and cyanide at p H 5.7 causes a relatively rapid drop in absorbance at 345 nm , a primary reaction followed by a slower secondary process. Comparison of the reactions of native and reconstituted hemocyanins with oxygen, fluoride, and cyanide shows that the reconstitution has been successful. Cadmium and manganese derivatives of hemocyanin have been prepared. The order of affinity of metals to the active site of hemocyanin is: Cu>Mn>Cd.