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Enzymatic Synthesis of Oligopeptide. Part II. Synthesis of Bis(N‐Protected Amino Acid) Hydrazides by Papain
Author(s) -
Chou ShanHo,
Wong ChiHuey,
Chen ShuiTien,
Wang KungTsung
Publication year - 1979
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.197900003
Subject(s) - chemistry , hydrazide , papain , hydrazine (antidepressant) , catalysis , derivative (finance) , amino acid , enzyme , peptide , organic chemistry , stereochemistry , medicinal chemistry , biochemistry , financial economics , economics
New hydrazide derivative identified as bis‐di‐N‐acylamino acid hydrazide was synthesized from N‐acylamino acid and hydrazine under papain catalysis. The reaction was carried out at pH 5.0, 38°C in a mixture containing 0.2M Z‐X‐OH + or Boc‐X‐OH, 2% NH 2 NH 2 ·H 2 O, 0.8% β‐mercaptoethanol, 0.1.% EDTA and 50 mg/ml of papain. The bis‐hydrazide can be used in peptide synthesis via oxidation.