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Enzymatic Synthesis of Oligopeptide. Part I. Papain‐Catalyzed Synthesis of Dipeptide, Tripeptide and Tetrapeptide
Author(s) -
Chou ShanHo,
Chen ShuiTien,
Wong ChiHuey,
Wang KungTsung
Publication year - 1978
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.197800035
Subject(s) - tetrapeptide , chemistry , papain , tripeptide , dipeptide , oligopeptide , stereochemistry , yield (engineering) , catalysis , peptide , enzyme , organic chemistry , biochemistry , materials science , metallurgy
Eighteen Z‐X‐Y‐OTNB + type dipeptides, four Z‐Val‐Cys(Bzl)‐X‐OTMB tripeptides and a tetrapeptide Z‐Val‐Cys(Bzl)‐Cys(Bzl)‐Asn‐OTMB have been synthesized by papain‐catalyzed reaction in reasonable yield after recystallization. Z‐DL‐Ala‐OH has been resolved through papain‐catalyzed synthesis of Z‐L‐Ala‐Leu‐OTMB with an optical purity as high as 98.6%. These results show that papain is a good catalyst for oligopeptide synthesis.