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Contribution of P‐O and P‐N Linkages in Phosphorylated Histone H1
Author(s) -
Wang L. Y.,
Fricke O.,
Lin Y. C.
Publication year - 1978
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.197800030
Subject(s) - chemistry , phosphorylation , polyethylene glycol , in vivo , histone , histone octamer , biochemistry , genetics , gene , nucleosome , biology
In studies of the phosphorylated proteins in Walker‐256 carcinosarcoma, it was established that the ratio of P‐N linkages to P‐O linkages on histone H 1 varied between 0.6 to 1.0. Histone H 1 was phosphorylated in vivo and isolated under all neutral conditions employing a dextran/polyethylene glycol two‐phase system and Bio‐Rex 70 chromatography.