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Amino Acid Sequence of N ‐Terminal Peptide of Normal Human Serum Albumin
Author(s) -
Liu C.S.,
Shih T. B.,
Hsin M.H.,
Blackwell R. Q.
Publication year - 1971
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.197100017
Subject(s) - thermolysin , chemistry , chymotrypsin , carboxypeptidase a , carboxypeptidase , trypsin , edman degradation , peptide , peptide sequence , amino acid , biochemistry , sequence (biology) , albumin , human serum albumin , bovine serum albumin , enzyme , gene
From the peptic digest of normal human serum albumin., the N ‐terminal peptide comprising 24 amino acid residues was obtained by means of peptide mapping. Combined uses of trypsin, α‐chymotrypsin, thermolysin, carboxypeptidase A and Dansyl‐Edman technique resulted in the elucidation of amino acid sequence of no. 1 to no. 24 as follows: NH 2 ‐Asp‐Ala‐His‐Lys‐Ser‐Glu‐Val‐Ala‐His‐Arg‐Phe‐Lys‐Asp‐Leu‐GIy‐Glu‐Glu‐Asn‐Phe‐Lys‐Ala‐Leu‐Val‐Leu‐COOH These sequence results agree completely with those recently published by other workers.