Premium
A blocking peptide stabilizes lysophosphatidic acid receptor 1 and promotes lysophosphatidic acid‐induced cellular responses
Author(s) -
Taleb Sarah J,
Wei Jianxin,
Mialki Rachel K,
Dong Su,
Li Yanhui,
Zhao Jing,
Zhao Yutong
Publication year - 2021
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.29919
Subject(s) - lysophosphatidic acid , ubiquitin ligase , microbiology and biotechnology , proteasome , ubiquitin , receptor , signal transduction , extracellular matrix , chemistry , biochemistry , biology , gene
G protein‐coupled receptors regulate a variety of cellular responses and have been considered as therapeutic targets for human diseases. Lysophosphatidic acid receptor 1 (LPA1) is a receptor for bioactive lysophospholipid, LPA. LPA/LPA1‐mediated signaling contributes to inflammatory and fibrotic responses in lung diseases; thus understanding regulation of LPA1 stability is important for modulating LPA/LPA1 signaling. Our previous study has shown that LPA1 is degraded in the Nedd4 like (Nedd4L) E3 ubiquitin ligase‐mediated ubiquitin‐proteasome system. In the current study, we attempt to identify a peptide that stabilizes LPA1 through disrupting LPA1 association with Nedd4L. LPA treatment induces both endogenous and overexpressed LPA1 degradation, which is attenuated by a proteasome inhibitor, suggesting that LPA1 is degraded in the proteasome. LPA increases phosphorylation of extracellular signal‐regulated kinase 1/2 (Erk1/2) and I‐κB kinase in lung epithelial cells, and this effect is promoted by overexpression of a peptide (P1) that mimics C‐terminal of LPA1. P1, not a control peptide, attenuates LPA‐induced LPA1 ubiquitination and degradation, suggesting that P1 stabilizes LPA1. Further, P1 diminishes Nedd4L‐mediated degradation of LPA1 and Nedd4L/LPA1 association. In addition to increasing LPA1 signaling, P1 enhances LPA‐induced cell migration and gene expression of Elafin , matrix metallopeptidase 1 , and serpin family B member 2 in lung epithelial cells. These data suggest that disruption of LPA1 interaction with Nedd4L by P1 increases LPA1 stability and LPA/LPA1 signaling.