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TBX2/3 is required for regeneration of dorsal‐ventral and medial‐lateral polarity in planarians
Author(s) -
Tian Qingnan,
Sun Yujia,
Gao Tingting,
Li Jiaxin,
Hao Zhitai,
Fang Huimin,
Zhang Shoutao
Publication year - 2021
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.29905
Subject(s) - planarian , gene knockdown , microbiology and biotechnology , rna interference , biology , ectopic expression , flatworm , morphogenesis , regeneration (biology) , anatomy , rna , gene , genetics , ecology
The molecular mechanisms responsible for axis establishment during non‐embryonic processes remain elusive. The planarian flatworm is an ideal model organism to study body axis polarization and patterning in vivo. Here, we identified a homolog of the TBX2/3 in the planarian Dugesia japonica . RNA interference (RNAi) knockdown of TBX2/3 results in the ectopic formation of protrusions in the midline of the dorsal surface which shows an abnormal expression of midline and ventral cell markers. Additionally, the TBX2/3 RNAi animals also show the duplication of expression of the boundary marker at the lateral edge. Furthermore, TBX2/3 is expressed in muscle cells and co‐expressed with bmp4 . Inhibition of bone morphogenetic protein (BMP) signaling reduces the expression of TBX2/3 at the midline. These results suggest that TBX2/3 RNAi results in phenotypic characters caused by inhibition of the BMP signal, indicating that TBX2/3 is required for DV and ML patterning, and might be a downstream gene of BMP signaling.