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GTPases Arf5 and Arl2 function partially distinctly during oocyte meiosis
Author(s) -
Zhou ChunXiang,
Wang Yang,
Shi LiYa,
Wang ZiBin,
Ma Yang,
Li CongRong,
Zhang NaNa,
Zhang YeXiao,
Zhang Fenli,
Zhang Dong,
Xia ZhengRong
Publication year - 2021
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.29839
Subject(s) - meiosis , microbiology and biotechnology , biology , oocyte , gtpase , meiosis ii , genetics , embryo , gene
Mammalian female meiosis must be tightly regulated to produce high‐quality mature oocytes for subsequent regular fertilization and healthy live birth of the next generation. GTPases control many important signal pathways involved in diverse cellular activities. ADP‐ribosylation factor family members (Arfs) in mice possess GTPase activities, and some members have been found to function in meiosis. However, whether other Arfs play a role in meiosis is unknown. In this study, we found that Arl2 and Arf5 are the richest among Arfs in mouse oocytes, and they are more abundant in oocytes than in granular cells. Furthermore, Arl2 and Arf5 depletion both impeded meiotic progression, but by affecting spindles and microfilaments, respectively. Moreover, Arl2 and Arf5 depletion both significantly increased regular reactive oxygen species levels and decreased mitochondrial membrane potential and autophagy, indicating that oocyte quality was damaged by Arl2 and Arf5 depletion. These results suggest that Arl2 and Arf5 are two novel essential GTPases required for oocyte meiosis and quality control.