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IRX5 prompts genomic instability in colorectal cancer cells
Author(s) -
Sun Xun,
Jiang Xinying,
Wu Jianzhong,
Ma Rong,
Wu Yiqi,
Cao Haixia,
Wang Zhuo,
Liu Siwen,
Zhang Junying,
Wu Yang,
Zhang Yuan,
Feng Jifeng,
Wang Ting
Publication year - 2020
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.29693
Subject(s) - colorectal cancer , homeobox , cyclin d1 , downregulation and upregulation , cancer , biology , genome instability , biomarker , cancer research , cell cycle , dna damage , gene , gene expression , genetics , dna
The Iroquois homeobox gene 5 (IRX5), one of the members of the Iroquois homeobox family, has been identified to correlate with worse prognosis in many cancers, including colorectal cancer (CRC). In this study, upregulation of IRX5 revealed a great reduction in the proliferation of CRC colorectal cancer cell line SW480 and DLD‐1, which was accompanied by G1/S arrest, increased expression in cyclin E1, P21, and P53 and a decrease in cyclin A2, B1, and D1. Furthermore, IRX5‐mediated an increase expression of RH2A protein, the biomarker of DNA damage. Consequently, the SA‐β‐gal level is higher in IRX5‐overexpression cells compared to control ones, which showed elevated DNA damage triggered cellular senescence. Recapitulating the above findings, IRX5 exhibited higher levels of genomic instability. IRX5 may be a perspective target for cancer therapy and it deserves further investigation.