Premium
MicroRNA‐7 promotes neural differentiation of trabecular meshwork mesenchymal stem cell on nanofibrous scaffold
Author(s) -
Jedari Behrouz,
Rahmani Ali,
Naderi Mahmood,
Nadri Samad
Publication year - 2020
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.29513
Subject(s) - nestin , mesenchymal stem cell , glial fibrillary acidic protein , microbiology and biotechnology , neural tissue engineering , chemistry , neural stem cell , stem cell , biomedical engineering , biology , immunology , immunohistochemistry , medicine , regeneration (biology)
The purpose of this study was to investigate miR‐7 overexpression effects on neural differentiation of mesenchymal stem cells (MSCs) on both two‐dimensional (2D) and three‐dimensional (3D) culture systems. We upregulated miR‐7 through lentiviral vector in trabecular meshwork MSCs (TMMSCs) and polymers of poly l ‐lactic acid/polycaprolactone fibrous scaffold were fabricated by electrospinning and characterized using scanning electron microscopy (SEM) and Fourier transform infrared (FTIR). Neural markers expression was evaluated through quantitative‐polymerase chain reaction (q‐PCR) and immunostaining. The results showed that the high percentage of cell transduction (84.9%) and miR‐7 expression (folds: 677.93 and 556.4) was detected in TMMSCs‐miR‐7(+). SEM and FTIR established the fabrication of the hybrid scaffold. q‐PCR analysis showed that on days 14 and 21 of transduction, the expression level of Nestin and glial fibrillary acidic protein (GFAP) genes were significantly higher in the scaffold (3D) compared with tissue culture polystyrene (2D) culture. The expression of microtubule‐associated protein‐2 (MAP‐2) and GFAP genes in TMMSCs‐miR‐7(+) cells were significantly higher than those miR‐7(−) cells after 21 days of cell culture. Also, MAP‐2 and Nestin proteins were detected in TMMSCs‐miR‐7(+) cells. Our results demonstrate that miR‐7 is involved in neural differentiation of TMMSCs and scaffold can improve differentiate into glial and neural progenitor cells. These findings provided some information for future use of microRNAs and scaffold in tissue engineering and cell therapy for neurological diseases.