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Retracted : Long noncoding RNA ZFPM2‐AS1 is involved in lung adenocarcinoma via miR‐511‐3p/AFF4 pathway
Author(s) -
Li Juan,
Ge Jun,
Yang Ye,
Liu Bin,
Zheng Min,
Shi Rui
Publication year - 2020
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.29476
Subject(s) - long non coding rna , non coding rna , biology , cancer research , microrna , lung , rna , genetics , gene , medicine
Lung cancer is the dominating cause of cancer‐induced death and can be classified into small cell lung cancer and non–small cell lung cancer (NSCLC). Lung adenocarcinoma (LUAD) is the most common histological subtype of NSCLC and its pathology remains unclear. Mounting reports have revealed that lncRNAs could regulate cellular activities in cancers. Yet the role of ZFPM2 antisense RNA 1 (ZFPM2‐AS1) in LUAD has not been elucidated. Using GEPIA online dataset, we identified the amplification of ZFPM2‐AS1 in LUAD tissues. Through quantitative real‐time reverse transcription‐polymerase chain reaction analysis, we observed an upregulation of ZFPM2‐AS1 in LUAD cell lines. Conducting loss‐of‐function assays, we found that ZFPM2‐AS1 depletion impaired cell viability, suppressed cell migration, and reversed epithelial‐mesenchymal transition progress in LUAD cells. Mechanism investigation manifested that ZFPM2‐AS1 was distributed in the cytoplasm of LUAD cells. Moreover, ZFPM2‐AS1 functioned as a molecular sponge of miR‐511‐3p, which was a suppressor in LUAD. Moreover, ZFPM2‐AS1 sponged miR‐511‐3p and thereby deregulated AF4/FMR2 family member 4 (AFF4), a target of miR‐511‐3p. At length, rescue assays indicated that AFF4 overexpression revived the inhibiting effects of ZFPM2‐AS1 knockdown on the biological processes in LUAD. All in all, this study uncovered the function and the mechanism of ZFPM2‐AS1 in LUAD.