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MiR‐3691‐5p is upregulated in docosahexaenoic acid‐treated vascular endothelial cell and targets serpin family E member 1
Author(s) -
Wu Yutang,
Chen Zhizhao,
Wang Yaoguo,
Peng Fangzhan
Publication year - 2020
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.29459
Subject(s) - docosahexaenoic acid , biology , downregulation and upregulation , serpin , microrna , apoptosis , microbiology and biotechnology , endothelial dysfunction , endocrinology , gene , genetics , biochemistry , polyunsaturated fatty acid , fatty acid
Endothelium, the inner cellular lining of blood vessels, has an important role in the regulation of physiological processes and its dysfunction may initiate cardiovascular complications. Previous investigations have revealed that dietary docosahexaenoic acid (DHA) is related to a lower possibility of cardiovascular disease and mortality. Until now, the molecular mechanisms in the biological activities of DHA remain largely unknown. MicroRNAs (miRNAs) play a vital role in regulating gene expression. Thus, we aimed to investigate whether DHA improves the dysfunction via regulating miRNAs. To understand the protective effects of DHA through modulating miR‐3691‐5p and its target genes for palmitic acid (PAL) induced apoptosis in endothelial cells. The present study demonstrated that DHA upregulated miR‐3691‐5p expression, and downregulated the expression of its target gene serpin family E member 1 ( SERPINE1 ). MiR‐mimics and inhibitors modulation results indicated that miR‐3691‐5p regulates endothelial apoptosis through activating antiapoptotic response which controlled by the STAT3 signaling pathway. In conclusion, we have shown that PAL‐induced apoptosis could be decreased by DHA treatment through miR‐3691‐5/SERPINE1 pathways.