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LncRNA AC009022.1 enhances colorectal cancer cells proliferation, migration, and invasion by promoting ACTR3B expression via suppressing miR‐497‐5p
Author(s) -
Yu Chen,
Zhang Fengchun
Publication year - 2020
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.29428
Subject(s) - colorectal cancer , transfection , cell growth , western blot , microrna , cancer research , cell , real time polymerase chain reaction , reverse transcription polymerase chain reaction , reporter gene , in vivo , microbiology and biotechnology , cancer , biology , gene expression , chemistry , medicine , cell culture , gene , biochemistry , genetics
Purpose Long noncoding RNAs (lncRNAs) play an indispensable role in cancer progression. We aim at exploring the effect of AC009022.1 on colorectal cancer (CRC) development in this paper. Methods CRC tissues and matched normal tissues of 68 CRC patients were collected. HCT‐116 and SW620 cells were transfected and grouped. The cell counting kit‐8 assay, cell scratch test, and Transwell experiment were performed to sequentially detect cells proliferation, migration, and invasion. In vivo experiments were conducted. The Luciferase reporter gene assay was used. Gene expression was detected by quantitative reverse transcription polymerase chain reaction and Western blot analysis. Results AC009022.1 expression was abnormally elevated in CRC tissues and cells. High AC009022.1 expression in CRC patients was significantly associated with poor prognosis. Compared with the siNC group, HCT‐116 and SW620 cells of siAC009022.1 group exhibited much lower OD 450 value ( P < .001) and invasive cell number ( P < .01), and significantly higher relative wound width ( P < .01). Much lower subcutaneous tumor volume and weight were found in the siAC009022.1 group compared with siNC group ( P < .001). In CRC cells, AC009022.1 directly inhibited miR‐497‐5p expression while miR‐497‐5p directly hindered ACTR3B expression. Compared with HCT‐116 and SW620 cells of oe‐AC009022.1 group, those of oe‐AC009022.1 + miR‐497‐5p‐mimics group and oe‐AC009022.1 + siACTR3B group had obviously lower OD 450 value ( P < .001) and invasion cell number ( P < .01), and markedly higher relative wound width ( P < .01). Conclusions AC009022.1 enhanced CRC cell proliferation, migration, and invasion by promoting ACTR3B expression via suppressing miR‐497‐5p.