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MicroRNA‐218‐5p relieves postmenopausal osteoporosis through promoting the osteoblast differentiation of bone marrow mesenchymal stem cells
Author(s) -
Kou Jianqiang,
Zheng Xiujun,
Guo Jianwei,
Liu Yang,
Liu Xiangyun
Publication year - 2020
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.29355
Subject(s) - runx2 , osteoblast , alkaline phosphatase , microrna , transfection , mesenchymal stem cell , postmenopausal osteoporosis , endocrinology , medicine , chemistry , osteoporosis , downregulation and upregulation , cancer research , microbiology and biotechnology , biology , bone mineral , biochemistry , enzyme , gene , in vitro
MicroRNAs (miRs) are short noncoding RNAs that play key regulatory roles in osteoblast differentiation. In this study, the specific regulatory roles of miR‐218‐5p on postmenopausal osteoporosis (PMOP) were investigated. The mouse model of PMOP was established by bilateral ovariectomy, and the injection of miR‐218‐5p mimics significantly relieved PMOP degree. Then, bone marrow mesenchymal stem cells (BMMSCs) isolated from PMOP mice were induced into osteoblasts. When compared with normal BMMSCs , PMOP BMMSCs exhibited significantly lower alkaline phosphatase (ALP) activity and less mineralized nodules, as well as downregulated miR‐218‐5p, Runx2, Osterix, COL1A1, and OCN after induction ( P < .05). The transfection of miR‐218‐5p mimics, and inhibitor significantly promoted, inhibited the osteoblast differentiation of PMOP BMMSCs, respectively. In addition, COL1A1 was a target of miR‐218‐5p. The transfection of miR‐218‐5p mimics into PMOP BMMSCs significantly upregulated COL1A1 at 14th and 21st day post‐induction, but not at 7th day. Our findings suggest miR‐218‐5p may relieve PMOP through promoting the osteoblast differentiation of BMMSCs.