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Downregulation of microRNA‐129‐5p increases the risk of intervertebral disc degeneration by promoting the apoptosis of nucleus pulposus cells via targeting BMP2
Author(s) -
Yang Weijie,
Sun Ping
Publication year - 2019
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.29274
Subject(s) - bone morphogenetic protein 2 , microrna , downregulation and upregulation , apoptosis , flow cytometry , cancer research , transfection , western blot , microbiology and biotechnology , intervertebral disc , chemistry , medicine , biology , anatomy , in vitro , biochemistry , gene
miR‐129‐5p is implicated in many diseases, such as laryngeal cancer and breast cancer. In this study, we studied the mechanism underlying the role of BMP2 in intervertebral disc degeneration (IDD). We used a luciferase assay system to determine the relationship between BMP2 and miR‐129‐5 expression. In addition, Western blot and real‐time PCR were used to confirm the regulatory relationship between miR‐129‐5p and its targets, while flow cytometry was used to evaluate the effect of miR‐129‐5p on the apoptosis of neural progenitor cells (NPCs). BMP2 was confirmed as a direct target of miR‐129‐5p. Furthermore, the expression of miR‐129 was downregulated along with upregulated BMP2 expression in IDD patients. Meanwhile, BMP2 was validated as the target of miR‐129‐5p in cells transfected with miR‐129‐5p and BMP2 siRNA. Also, compared with NPCs transfected with blank/scramble controls or miR‐129‐5p inhibitors, the NPCs treated with miR‐129‐5p mimics or BMP2 siRNA exhibited evidently elevated viability and inhibited apoptosis. The data demonstrated that miR‐129‐5p was poorly expressed in IDD patients, and the dysregulation of miR‐129‐5p might contribute to the development of IDD by targeting BMP2 expression.