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Evaluation of the effect of cafeteria diet on the kidney Na,K‐ATPase activity, and oxidative stress
Author(s) -
Pinheiro Isadora Reis Restier,
Melo Marina Fátima Nunes,
Sousa Sarah Vivas,
Cardoso Bárbara Gatti,
da Silva Thaís Marques,
Rangel Luciana Pereira,
Cortes Vanessa Faria,
Lima Santos Hérica,
Chaves Valéria Ernestânia,
Garcia Israel José Pereira,
Barbosa Leandro Augusto
Publication year - 2019
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.29228
Subject(s) - medulla , endocrinology , medicine , oxidative stress , renal cortex , cortex (anatomy) , chemistry , glutathione peroxidase , lipid peroxidation , kidney , glutathione , glutathione reductase , enzyme , biology , biochemistry , superoxide dismutase , neuroscience
In this study, renal tissue, subdivided into the cortex and medulla of Wistar rats subjected to a cafeteria diet (CAF) for 24 days or to normal diet, was used to analyze whether the renal enzyme Na,K‐ATPase activity was modified by CAF diet, as well as to analyze the α1 subunit of renal Na,K‐ATPase expression levels. The lipid profile of the renal plasma membrane and oxidative stress were verified. In the Na,K‐ATPase activity evaluation, no alteration was found, but a significant decrease of 30% in the cortex was detected in the α1 subunit expression of the enzyme. There was a 24% decrease in phospholipids in the cortex of rats submitted to CAF, a 17% increase in cholesterol levels in the cortex, and a 23% decrease in the medulla. Lipid peroxidation was significantly increased in the groups submitted to CAF, both in the cortical region, 29%, and in the medulla, 35%. Also, a reduction of 45% in the glutathione levels was observed in the cortex and medulla with CAF. CAF showed a nearly two‐fold increase in glutathione peroxidase (GPX) activity in relation to the control group in the cortex and a 59% increase in the GPx activity in the medulla. In conclusion, although the diet was administered for a short period of time, important results were found, especially those related to the lipid profile and oxidative stress, which may directly affect renal function.