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Protective effect of RNA interference targeting Ikkβ on hepatic fibrosis in rats
Author(s) -
Wei ShiFang,
Sun JianJun,
Zhou Ning,
Li XiangLin,
Lin Yan,
Zhang YaoDi,
Zhang YueRong,
Wu LiYang
Publication year - 2019
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.29209
Subject(s) - iκb kinase , nf κb , inflammation , fibrosis , iκbα , cancer research , small interfering rna , signal transduction , transcription factor , hepatic stellate cell , rna interference , tumor necrosis factor alpha , chemistry , biology , microbiology and biotechnology , transfection , immunology , medicine , endocrinology , biochemistry , gene , rna
Nuclear factor‐κB (NF‐κB) is an important regulatory factor in cells. NF‐κB has a wide range of biological activities. After activation, it participates in the transcription and regulation of many genes and plays a role in infection, inflammatory response, oxidative stress, cell multiplication, and apoptosis. The activation of the NF‐κB signal pathway is dependent on the degradation of the IκB kinase β (IKKβ) complex. IKK β is the key kinase in the NF‐κB activation pathway. After inhibition, it can block the activation of NF‐κB. IKKβ is a key regulator of NF‐κB activation, also an early regulator of inflammation in all stages of the immune response. This study aimed to investigate the effect of IKKβ‐siRNA lentivirus vector treatment for hepatic fibrosis of rats. An IKKβ‐siRNA expression plasmid was constructed and injected in the tail vein of rats. Then, IKKβ‐siRNA distribution in the liver was observed by immunofluorescence, and the quantitative polymerase chain reaction was used to detect inflammation‐related and fibrosis‐related factors. IKKβ‐siRNA lentiviruses could be delivered to the liver and significantly decrease carbon tetrachloride‐induced hepatic fibrosis. Furthermore, serum transaminase levels significantly decreased, and inflammation‐related and fibrosis‐related factors decreased. IKKβ‐siRNA can be an effective method of anti‐fibrosis gene therapy for liver fibrosis.