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Aerobic training induces differential expression of genes involved in lipid metabolism in skeletal muscle and white adipose tissues
Author(s) -
Souza Cordeiro Letícia Maria,
Mario Érica Guilhen,
Moreira Carolina Campos Lima,
Rodrigues Angélica Heringer,
Wanner Samuel Penna,
Soares Danusa Dias,
Botion Leida Maria,
Ferreira Adaliene Versiani Matos
Publication year - 2019
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.29208
Subject(s) - adipose tissue , endocrinology , medicine , white adipose tissue , citrate synthase , lipid metabolism , skeletal muscle , carnitine , aerobic exercise , biology , biochemistry , enzyme
Aerobic training induces adaptive responses in skeletal muscles and white adipose tissues, thus facilitating lipid utilization as energy substrates during a physical exercise session. However, the effects of training on cytokines levels and on transcription factors involved in lipid metabolism in muscle and different white adipose depots are still unclear; therefore, these were the aims of the present study. Nineteen adult male Wistar rats were randomly assigned to a trained group or a control, non‐trained group. The 10‐week training protocol consisted of running on a treadmill, during 1 hour per day, 5 days per week, at 75% of maximum aerobic speed. As expected, trained rats improved their aerobic performance and had augmented citrate synthase activity in the soleus, while the control rats did not. Although body weight was not different between groups, the adiposity index and white adipose depots (ie, epididymal and retroperitoneal) were reduced in trained rats. Training reduced serum concentration of insulin, but failed to change serum concentrations of glucose, triacylglycerol, total cholesterol, and nonesterified fatty acids. Training increased sterol regulatory element‐binding protein‐1c expression in the gastrocnemius and epididymal adipose tissue, and reduced peroxisome proliferator‐activated receptor γ (PPARγ) expression in most of the tissues analyzed. The expression of PPARα and carnitine palmitoyltransferase 1 increased in the gastrocnemius and mesenteric adipose tissue but reduced in epididymal adipose tissue. Triacylglycerol content and tribbles 3 expression reduced in the gastrocnemius of trained rats. Tumor necrosis factor‐α and interleukin‐6 were increased in all adipose depots evaluated. Collectively, our data indicate that the 10‐week aerobic training changed gene expression to improve muscle oxidative metabolism and facilitate lipid degradation in adipose tissues. Our data also highlight the existence of adaptive responses that are distinct between the skeletal muscle and white adipose tissue and between different adipose depots.