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C allele of ‐786 T>C polymorphism in the promoter region of endothelial nitric oxide synthase is responsible for endothelial dysfunction in the patients with rheumatoid arthritis
Author(s) -
Luo Yanli,
Wang Yang,
Luo Wanjun
Publication year - 2020
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.29184
Subject(s) - enos , brachial artery , endothelial dysfunction , genotype , allele , nitric oxide synthase , microbiology and biotechnology , luciferase , nitric oxide , medicine , western blot , biology , immunology , endocrinology , andrology , transfection , gene , genetics , blood pressure
Abstract Background This study aimed to explore the roles of endothelial nitric oxide synthase (eNOS) in the control of metastasis of infection with endothelial dysfunction, as well as the roles of ‐786T>C polymorphism in eNOS promoter in the control of metastasis of endothelial function. Method In‐silicon analysis and luciferase assay were used to identify the location of ‐786>C on the promoter of eNOS. Subsequently, real‐time PCR and Western‐blot were used to determine the expression level of eNOS. Ultrasound examination was used to detect baseline brachial artery diameter and flow‐mediated dilation of patients in different treat groups. Results ‐786T>C was located on the promoter of eNOS, and the luciferase activity of cells transfected with ‐786‐C allele was much higher than empty vector, while even higher subsequent to transfection of ‐786‐T allele. In addition, the result of ultrasound examination showed that the baseline brachial artery diameter was comparable between patients genotyped as TT, TC and CC, while the flow‐mediated dilation of patients genotyped as TC was much higher compared with CC group, and the flow‐mediated dilation of patients genotyped as TT even higher than TC group. We found eNOS messenger RNA and protein with TT genotype was significantly higher compared with other genotypes. And the production of NO was remarkably higher in TT groups compared with TC and CC, while the production of NO in TC and CC groups were similar. Conclusion These findings indicated that down‐expression of ‐786T>C located on the promoter of eNOS is associated with an increased risk of endothelial dysfunction.