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Upregulation of microRNA‐196a improves cognitive impairment and alleviates neuronal damage in hippocampus tissues of Alzheimer's disease through downregulating LRIG3 expression
Author(s) -
Yang Ke,
Feng Shutao,
Ren Jun,
Zhou Wenbin
Publication year - 2019
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.29047
Subject(s) - downregulation and upregulation , pi3k/akt/mtor pathway , hippocampus , hippocampal formation , protein kinase b , oxidative stress , neuroprotection , neuroscience , endocrinology , medicine , biology , signal transduction , microbiology and biotechnology , biochemistry , gene
Purpose This study is launched to uncover the inner function of microRNA‐196a (miR‐196a) on cognitive dysfunction and neuronal damage in Alzheimer's disease (AD) rats through regulating the PI3K/Akt signaling pathway. Methods The establishment of AD rat model was performed by a microinjection of Aβ 25‐35 . miR‐196a and LRIG3 expression was detected, and the putative binding site between them was also determined. The spatial learning and memory capability, the hippocampal neurons ultrastructure as well as the survival, and apoptosis of hippocampal neurons of rats were observed. The expression of apoptosis‐associated protein, oxidative stress index, and inflammatory factors as well as the PI3K/Akt pathway‐related factors was determined. Results Initially, decreased miR‐196a and increased LRIG3 were exhibited in hippocampus tissues of AD rats. In addition, restored miR‐196a and deleted LRIG3 ameliorated spatial learning and memory capability, suppressed the pathological injury, induced the survival, and suppressed the apoptosis of hippocampal neurons, as well as inhibited oxidative stress injury together with inflammatory injury in AD rats. Furthermore, upregulation of miR‐196a activated the PI3/Akt pathway in AD rats. Conclusion This current study suggests that upregulation of miR‐196a and downregulation of LRIG3 improve cognitive impairment and alleviate neuronal damage in hippocampus tissues in AD rats via the modulation of the PI3K/Akt pathway.