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Titanium with nanotopography induces osteoblast differentiation through regulation of integrin αV
Author(s) -
Lopes Helena Bacha,
Freitas Gileade Pereira,
Fantacini Daianne Maciely Carvalho,
PicançoCastro Virginia,
Covas Dimas Tadeu,
Rosa Adalberto Luiz,
Beloti Marcio Mateus
Publication year - 2019
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.28930
Subject(s) - nanotopography , integrin , microbiology and biotechnology , osteoblast , cell adhesion , chemistry , cellular differentiation , signal transduction , downregulation and upregulation , mechanotransduction , adhesion , cell , biology , gene , biochemistry , in vitro , organic chemistry
Topographical modifications of titanium (Ti) at the nanoscale level generate surfaces that regulate several signaling pathways and cellular functions, which may affect the process of osseointegration. Here, we investigated the participation of integrin αV in the osteogenic capacity of Ti with nanotopography. Machined titanium discs (untreated) were submitted to treatment with H 2 SO 4 /H 2 O 2 to produce the nanotopography (nanostructured). First, the greater osteogenic capacity of the nanotopography that increased osteoblast differentiation of mesenchymal stem cells compared with untreated topography was shown. Also, the nanostructured surface increased (regulation ≥ 1.9‐fold) the gene expression of 6 integrins from a custom array plate utilized to evaluate the gene expression of 84 genes correlated with cell adhesion signaling pathway, including integrin αV, which is involved in osteoblast differentiation. By silencing integrin αV in MC3T3‐E1 cells cultured on nanotopography, the impairment of osteoblast differentiation induced by this surface was observed. In conclusion, it was shown that nanotopography regulates the expression of several components of the cell adhesion signaling pathway and its higher osteogenic potential is, at least in part, due to its ability to upregulate the expression of integrin αV. Together with previous data that showed the participation of integrins α1, β1, and β3 in the nanotopography osseoinduction activity, we have uncovered the pivotal role of this family of membrane receptors in the osteogenic potential of this surface.