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Increased expression of miR‐146a, miR‐10b, and miR‐21 in cancer stem‐like gastro‐spheres
Author(s) -
Bakhshi Mahdieh,
Asadi Jahanbakhsh,
Ebrahimi Marzieh,
Moradi AbdolVahab,
Hajimoradi Monireh
Publication year - 2019
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.28918
Subject(s) - downregulation and upregulation , microrna , cancer stem cell , cancer research , biology , stem cell , docetaxel , reverse transcription polymerase chain reaction , cancer cell , cancer , microbiology and biotechnology , gene expression , gene , genetics
Background Gastric cancer remains one of the leading causes of cancer‐associated mortalities globally. Accumulating evidence support the presence of gastric cancer stem cells (CSCs) and their role in the pathogenesis and therapeutic challenges of gastric cancer. MicroRNAs (miRNAs) may be influenced by the cellular differentiative state and as critical regulators of the cellular fate in development and cancer, can modulate the behavior of CSCs too. Here, we aimed to investigate the expression relevance of three prognostic miRNAs (miR‐21, miR‐10b, and miR‐146a) in CSCs of AGS and MKN‐45 gastric cancer cell lines. Methods Serial sphere‐forming assay in serum‐free culture medium was used to enrich the cellular population with stem‐like properties. Gastro‐spheres were characterized by evaluating the stemness gene expression, clonogenicity, and resistance to docetaxel and cisplatin in comparison with their parental cells. The expression level of miRNAs in gastro‐spheres and their parental cells was measured using quantitative reverse transcription polymerase chain reaction. Results Gastro‐spheres from both cell lines exhibit stem‐like properties: upregulated stemness associated genes ( P < 0.05), more colonogenicity and more resistance to docetaxel ( P < 0.05). MKN‐45 gastro‐spheres exhibited upregulated expression of miR‐21 (1.8‐folds), miR‐10b (1.34‐folds) and miR‐146a (4.8‐folds; P < 0.05) compared with the parental cells. AGS‐derived gastro‐spheres showed upregulation of miR‐21 (4.7‐folds; P < 0.01), miR‐10b (15.2‐folds; P < 0.001) and miR‐146a (39.3‐folds; P < 0.05). Conclusion Our data exhibited upregulation of miR‐21, miR‐10b, and miR‐146a in the stem‐like gastro‐spheres; however; their function in gastric CSCs remains to be verified by further experiments.