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Overexpression of circ_0005198 sponges miR‐1294 to regulate cell proliferation, apoptosis, migration, and invasion in glioma
Author(s) -
Wang Jiandong,
Li Jie,
Wang Hongwei,
Lv Luting,
Sun Jinghui
Publication year - 2019
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.28820
Subject(s) - apoptosis , cancer research , glioma , malignancy , cell growth , clone (java method) , biology , luciferase , downregulation and upregulation , cell culture , transfection , gene , genetics
Glioma (GM) is an aggressive malignancy with high mortality rate across the world. Mounting studies demonstrates that abnormally expressed circular RNAs (circRNAs) act as important roles in tumor progression. In the current work, a novel circRNA, circ_0005198, was explored. Circ_0005198 level in GM tissue samples and cells was detected. The clinical implication of circ_0005198 was analyzed by Fisher's test and Kaplan‐Meier analysis. In vitro experiments were carried out to elucidate the influence of circ_0005198 on GM cell proliferation, apoptosis, and metastatic properties. Luciferase reporter and rescue experiments were conducted to clear the mechanism of circ_0005198. The expression of circ_0005198 was enhanced in GM tumors and cells. Its expression in tumor specimens was related to clinical severity and poor prognosis. Functionally, circ_0005198 could remarkably boost cell growth, clone‐forming ability, and metastatic properties and attenuate cell apoptosis in GM cells. What's more, circ_0005198 could directly sponge miR‐1294 to exert oncogenic functions. In summary, this study might provide an effective therapeutic target for GM.