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Knockdown of LASP2 inhibits the proliferation, migration, and invasion of cervical cancer cells
Author(s) -
Zhang Yimeng,
Zhang Liya
Publication year - 2019
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.28806
Subject(s) - gene knockdown , cancer research , microbiology and biotechnology , cell migration , cell growth , cervical cancer , chemistry , biology , cancer , cell , medicine , apoptosis , biochemistry
LIM and SH3 protein 2 (LASP2) belongs to nebulin family. It has been proven that LASP2 is involved in several cancers; however, its role in cervical cancer is unclear. Herein, we showed that LASP2 was highly expressed in cervical cancer tissues and cell lines. To knockdown LASP2 in cervical cancer cells, small interfering RNAs (siRNAs) targeting LASP2 (si‐LASP2) were used. We found that cell proliferation, migration/invasion were markedly reduced after si‐LASP2 transfection. A significant increase in E‐cadherin expression, and decrease in N‐cadherin and vimentin expressions were observed in si‐LASP2 transfected cervical cancer cells. Knockdown of LASP2 caused significant inhibitory effect on the PI3K/Akt pathway. Treatment with the activator of the PI3K/Akt pathway, 740Y‐P, abolished the effects of si‐LASP2 transfection on cervical cancer cells. These findings suggested that LASP2 may be an oncogene through regulating the PI3K/Akt pathway in cervical cancer.