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3,3′,4,4′,5‐Pentachlorobiphenyl influences mitochondrial apoptosis pathway in granulosa cells
Author(s) -
Zhong Tao,
Zhang Jianmei,
Han Xiaoying,
Gongye Xiaoxiao,
Lyu Tianqi,
Jiang Menghan,
Yu Kaiwei,
Meng Xiaoqian,
Cheng Dong,
Lyu Hui,
Zhang Tianliang,
Zhang Lei,
Liu Shuzhen
Publication year - 2019
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.28801
Subject(s) - apoptosis , microbiology and biotechnology , chemistry , andrology , polychlorinated biphenyl , biology , biochemistry , medicine , environmental chemistry
3,3′,4,4′,5‐Polychlorinated biphenyl (PCB126) is a persistent organic environmental pollutant which can affect various biological activities of organisms, such as immunity, neurological function, and reproduction. In our study, we aimed to investigate the effects of PCB126 on granulosa cells (GCs). GCs were collected from ovaries in PMSG‐treated mice, after 24 hours culture. GCs were then incubated with 10 pg/mL, 100 pg/mL, and 10 ng/mL of PCB126 for another 24 hours. Following these steps, exposed GCs were collected for further experimentation. Our data showed that the number of GCs in the 10 ng/mL PCB126 decreased. Meanwhile, pyknotic nuclei and condensed chromatin increased, while the apoptotic cells in the 10 ng/mL PCB126 group were significantly increased. Furthermore, the expression of the apoptotic executive protein caspase‐3 increased after PCB126 treatment. The expression of Bax, Bcl‐2, and Bim related to the mitochondrial apoptosis pathway were also influenced to different degrees. Thus, our data suggested that PCB126 affect the GCs apoptosis, and mitochondrial apoptosis pathway was involved in this process.