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microRNA‐199a‐5p suppresses glioma progression by inhibiting MAGT1
Author(s) -
Wang Guang,
Li Yong,
Li Jie,
Zhang Dongxia,
Luo Chao,
Zhang Bingqian,
Sun Xiaochuan
Publication year - 2019
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.28791
Subject(s) - microrna , glioma , cancer research , medicine , neuroscience , chemistry , microbiology and biotechnology , biology , gene , biochemistry
microRNAs (miRNAs) can function as a tumor suppressor or oncogenic genes in human cancers. Alternation expression of miR‐199a‐5p has been revealed in several human cancers. However, its expression pattern and biological roles in glioma remain unclear. Expression levels of miR‐199a‐5p in glioma were evaluated at first. The effects of miR‐199a‐5p expression on cell proliferation, migration, and invasion were investigated using the MTT assay, wound‐healing assay, and transwell invasion assay. The expression of miR‐199a‐5p was found to be reduced in glioma cell lines. Overexpression of miR‐199a‐5p inhibits glioma cell proliferation, migration, and invasion in vitro. Furthermore, the target of miR‐199a‐5p was predicted by TargetScan and validated by luciferase activity reporter assay. We found magnesium transporter 1 (MAGT1) was a direct target of miR‐199a‐5p. Overexpression of MAGT1 reversed the effects of miR‐199a‐5p on glioma cell behaviors. Taken together, our study revealed that miR‐199a‐5p and MAGT1 have the potential to be used as a biomarker for glioma.