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Expression of miR‐486‐5p and its significance in lung squamous cell carcinoma
Author(s) -
Yang Sheng,
Sui Jing,
Liu Tong,
Wu Wenjuan,
Xu Siyi,
Yin Lihong,
Pu Yuepu,
Zhang Xiaomei,
Zhang Yan,
Shen Bo,
Liang Geyu
Publication year - 2019
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.28665
Subject(s) - pten , biology , lung cancer , microrna , cancer research , real time polymerase chain reaction , oncology , biomarker , gene expression , carcinoma , gene expression profiling , confidence interval , gene , medicine , apoptosis , genetics , pi3k/akt/mtor pathway
Lung squamous cell carcinoma (LUSC) is one of the main histological types of lung cancer with high mortality. The role of microRNA‐486‐5p in LUSC remains unclear. In the current study, the aim was to explore miR‐486‐5p expression and its role in LUSC. The miR‐486‐5p expression was significantly low‐expressed in patients with LUSC from The Cancer Genome Atlas database, which was further confirmed in the Gene Expression Omnibus database, patients’ tissues, different cell lines by quantitative real‐time polymerase chain reaction, and the high‐throughput gene sequencing data of lung tissues of mice after a long‐term B(a)P exposure. The meta‐analysis was performed to evaluate the expression and diagnosis power of miR‐486‐5p (standard mean difference = −2.25; 95% confidence interval: −3.47 to −1.03; P = 0.0003; area under curve = 0.9082). Functional enrichment analysis revealed the potential function of miR‐486‐5p in LUSC using gene set enrichment analysis and clusterProfiler package in R software. At last, the hub genes (PTEN, TEK, PIK3R1, PPM1B, SMAD2, and SPTA1) of miR‐486‐5p were verified. In conclusion, miR‐486‐5p may be a LUSC antioncogene, playing an important role to serve as a biomarker in LUSC.