Premium
Identify the critical protein‐coding genes and long noncoding RNAs in cardiac myxoma
Author(s) -
Cheng Nan,
Wu Yuanbin,
Zhang Huajun,
Guo Yi,
Cui Huimin,
Wei Shixiong,
Zhao Yuancheng,
Wang Rong
Publication year - 2019
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.28618
Subject(s) - gene , biology , coding (social sciences) , non coding rna , computational biology , genetics , microrna , sociology , social science
Abstract Cardiac myxoma (CM) is the most common benign cardiac tumor which is mostly sporadic. Increasing evidence show that protein‐coding genes (PCGs) and long noncoding RNAs (lncRNAs) play important roles in the pathology processes of multiple cancers. However, the functional roles and regulatory mechanisms of RNAs interaction in CM are still unclear. In this study, we investigated three pairs of surgically excised CM by high throughput sequencing and screened a set of PCGs and lncRNAs which were differentially expressed and could serve as expression markers in CM. By constructing protein‐protein interactions (PPI) and lncRNA‐mRNA coexpressing network, we screened out a CM‐related hub lncRNA‐mRNA modules, which were enriched in different pathways such as MAPK and TGF‐beta whose imbalance were validated by q‐PCR. In addition, we identified a specific dysregulated competing endogenous RNA (ceRNA) network in CM by integrating lncRNA‐miRNA‐mRNA interactions. These results will help us to understand the interaction mechanisms of RNAs in CM and provide novel PCGs and lncRNAs as potential therapeutic targets for CM.