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Cryptotanshinone inhibits hypoxia/reoxygenation‐induced oxidative stress and apoptosis in renal tubular epithelial cells
Author(s) -
Zhu Rui,
Wang Wei,
Yang Suxia
Publication year - 2019
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.28609
Subject(s) - salvia miltiorrhiza , pi3k/akt/mtor pathway , protein kinase b , oxidative stress , apoptosis , reactive oxygen species , ly294002 , chemistry , viability assay , pharmacology , superoxide dismutase , hypoxia (environmental) , phosphatidylinositol , medicine , kinase , biochemistry , pathology , oxygen , alternative medicine , organic chemistry , traditional chinese medicine
Cryptotanshinone (CTS), an active component extracted from the root of Salvia miltiorrhiza Bunge , was reported to attenuate hepatic ischemia/reperfusion (I/R) injury. However, its protective effect against renal I/R injury remains unclear. In this study, the role of CTS in renal I/R injury in vitro and its possible mechanism were investigated. Our results showed that CTS improved cell viability in HK‐2 cells exposed to hypoxia/reoxygenation (H/R). CTS also inhibited the H/R‐mediated production of reactive oxygen species, as well as increased the activities of superoxide dismutase and catalase in H/R‐stimulated HK‐2 cells. In addition, CTS dramatically attenuated the induction of bax expression and caspase‐3 activity and alleviated the reduction of bcl‐2 expression in HK‐2 cells cultured with H/R. Furthermore, CTS activated the levels of p‐PI3K and p‐Akt in H/R‐injured HK‐2 cells; meanwhile, the renal protective activity of CTS was inhibited by the inhibitor of the (phosphatidylinositol 3 kinase/protein kinase B) PI3K/Akt pathway (LY294002). These findings indicate that CTS can ameliorate renal I/R injury in vitro partly through regulating the PI3K/Akt pathway.