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Exogenous noggin binds the BMP‐2 receptor and induces alkaline phosphatase activity in osteoblasts
Author(s) -
Hashimi Saeed M.
Publication year - 2019
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.28597
Subject(s) - noggin , alkaline phosphatase , bone morphogenetic protein , chemistry , bone morphogenetic protein 2 , osteoblast , microbiology and biotechnology , medicine , endocrinology , bone morphogenetic protein 7 , biology , biochemistry , enzyme , gene , in vitro
Abstract Osteogenesis is an important process in bone remodeling and is under strict cellular signaling governed by growth factors and antagonists. Bone morphogenetic protein 2 (BMP‐2) is an important osteogenic factor involved in the transcription of key osteogenic genes such as alkaline phosphatase (ALP). While, antagonists such as noggin effectively restrict osteoblast differentiation through binding to BMP‐2. In this study, we sought to understand the effect of exogenous noggin in osteoblasts and its role in BMP‐2 activation of osteogenesis. Enzymatic activity of ALP was monitored to ascertain the effect of the noggin. Fluorescently labelled noggin was used to determine the binding of noggin to the BMP‐2 receptor. The results demonstrated that noggin significantly increases the activity of ALP at concentrations of 50 to 400 ng/mL. While, it inhibited the activity of exogenous BMP‐2. Furthermore, fluorescently labelled noggin showed strong binding to osteoblasts which were perturbed when cells were preincubated with BMP‐2 suggesting that noggin shares a common receptor with BMP‐2. These results suggest that exogenous noggin facilitates osteogenic differentiation and provide a novel mechanism for its interplay with BMP‐2.