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Stimulatory effects of platycodin D on osteoblast differentiation
Author(s) -
Han Younho,
Jin Sun Woo,
Lee Gi Ho,
Choi Jae Ho,
Lee Hyun Sun,
Chung Young Chul,
Jeong Hye Gwang,
Lee Kwang Youl
Publication year - 2019
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.28580
Subject(s) - osteoblast , chemistry , osteoclast , runx2 , alkaline phosphatase , bone sialoprotein , microbiology and biotechnology , downregulation and upregulation , mineralization (soil science) , osteocalcin , biochemistry , biology , receptor , enzyme , in vitro , organic chemistry , gene , nitrogen
Previous studies have suggested that platycodin D is implicated in bone biology and ameliorates osteoporosis development. Platycodin D repressed the osteoclast activity and enhanced bone mineral density in the mouse model. However, the effects of platycodin D on osteoblast differentiation have not been elucidated yet. In C3H10T1/2 cells, platycodin D upregulated osteogenic markers including alkaline phosphatase (ALP), bone sialoprotein, and collagen type 1 alpha 1, and transcription factors, such as Runx2 and osterix, subsequently enhancing the bone mineralization. In a molecular mechanism study, platycodin D induced β‐catenin nuclear accumulation by upregulating GSK3β phosphorylation. Furthermore, platycodin D upregulated the ALP activity and enhanced the mineralization process in osteoblast cells via the sirtuin 1/β‐catenin pathways. Taken together, these results suggested that platycodin D could be an effective therapeutic compound against osteoporosis because of its regulatory effects during the osteoblast differentiation.