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Long noncoding RNA SNHG12 promotes cell proliferation and activates Wnt/β‐catenin signaling in prostate cancer through sponging microRNA‐195
Author(s) -
Song Jiannan,
Wu Xuhong,
Ma Rong,
Miao Ling,
Xiong Lei,
Zhao Wei
Publication year - 2019
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.28578
Subject(s) - wnt signaling pathway , gene knockdown , long non coding rna , competing endogenous rna , microrna , cancer research , downregulation and upregulation , cell growth , biology , prostate cancer , microbiology and biotechnology , signal transduction , cancer , cell culture , genetics , gene
Long noncoding RNAs (lncRNAs) serve critical roles in multiple human malignant tumors, including prostate cancer (PCa). Currently, the biological role of oncogenic lncRNA SNHG12 in PCa remains largely unclear. In the present study, we found that SNHG12 was highly expressed in human PCa tissues and cell lines. In addition, gain‐of‐function and loss‐of‐function studies showed that overexpression of SNHG12 promoted, while downregulation suppressed the proliferation, invasion, and migration of PCa cells in vitro. Knockdown of SNHG12 also repressed PCa xenograft tumor growth in vivo. Further in‐depth mechanistic studies showed that SNHG12 might serve as a competing endogenous RNA for miR‐195 in PCa cells, and miR‐195 expression level was negatively associated with the expression of SNHG12 in PCa tissues. Finally, we found that the activity of Wnt/β‐catenin signaling is enhanced by SNHG12 overexpression and rescued by co‐transfection with miR‐195 mimics in PCa cells. Collectively, the present study indicated the oncogenic function of SNHG12 in PCa and our findings might provide a new target in the treatment of PCa.