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Long noncoding RNA TALNEC2 regulates myocardial ischemic injury in H9c2 cells by regulating miR‐21/PDCD4‐medited activation of Wnt/β‐catenin pathway
Author(s) -
Hao Lin,
Wang Juan,
Liu Na
Publication year - 2019
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.28562
Subject(s) - downregulation and upregulation , hypoxia (environmental) , wnt signaling pathway , catenin , microbiology and biotechnology , long non coding rna , apoptosis , microrna , cancer research , biology , signal transduction , chemistry , gene , genetics , organic chemistry , oxygen
The goal of this study was to explore the role of tumor associated long noncoding RNA expressed on chromosome 2 (TALNEC2) in protecting against myocardial ischemic injury, as well as its underlying molecular mechanism. We established a cell model of myocardial injury through treating H9c2 cells with hypoxia, and the expression level of TALNEC2 was analyzed. Further, in vitro studies investigated the functional role of TALNEC2 dysregulation in hypoxia injury by assessing cell proliferation, migration, invasion, and apoptosis. Moreover, the expression of miR‐21 was determined after dysregulation of TALNEC2, and whether TALNEC2‐regulated hypoxia injury in H9c2 cells via regulating miR‐21 expression were explored. Furthermore, the regulatory relationship between TALNEC2 and Wnt/β‐catenin pathway was also investigated. TALNEC2 was highly expressed in the serum from patients with myocardial ischemic compared with that in healthy persons. Hypoxia‐induced injury in H9c2 cells. Overexpression of TALNEC2 aggravated hypoxia injury in H9c2 cells. TALNEC2 could negative regulate the miR‐21 expression, and overexpression of TALNEC2 aggravated hypoxia injury by downregulation of miR‐21. Moreover, miR‐21 negatively regulated the PDCD4 expression, and PDCD4 was a target of miR‐21. Further studies disclosed that the overexpression of TALNEC2 further activated the Wnt/β‐catenin pathway in hypoxia‐treated H9c2 cells, implying that the Wnt/β‐catenin pathway was a downstream mechanism mediating the role of TALNEC2 in regulating hypoxia injury in H9c2 cells. These findings confirmed the key functions of TALNEC2 in regulating myocardial ischemic injury. Upregulation of TALNEC2 may aggravate hypoxia injury in H9c2 cells via regulating miR‐21/PDCD4‐medited activation of the Wnt/β‐catenin pathway. TALNEC2 may serve as a promising therapeutic target in myocardial ischemia.