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Upregulation of hsa_circ_0136666 contributes to breast cancer progression by sponging miR‐1299 and targeting CDK6
Author(s) -
Liu LiHong,
Tian QingQing,
Liu Juan,
Zhou Yong,
Yong Hongmei
Publication year - 2019
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.28536
Subject(s) - cyclin dependent kinase 6 , downregulation and upregulation , breast cancer , cancer research , cancer , microrna , cell cycle , cell growth , tumor progression , medicine , chemistry , oncology , cyclin d1 , gene , biochemistry
Circular RNAs (circRNAs) can participate in multiple cancers, including breast cancer. Increasing circRNAs are recognized in various cancers because of the high‐throughput sequencing. However, the potential physiological effect of hsa_circ_0136666 in breast cancer progression is unknown. In our study, the biological role of hsa_circ_0136666 in breast cancer development was studied. It was displayed that hsa_circ_0136666 was greatly increased in breast cancer. In addition, overexpression of hsa_circ_0136666 was able to promote Michigan Cancer Foundation‐7 (MCF7) and BT474 cell proliferation and triggered cell cycle in G2/M phase. microRNA plays critical role in tumor development and they can act as direct targets of circRNAs. miR‐1299 has been implicated as a famous tumor suppressor in many cancers. Here, miR‐1299 was predicted as the target of hsa_circ_0136666. Meanwhile, its Upregulation repressed breast cancer proliferation, migration and invasion capacity, which could be reversed by the increase of hsa_circ_0136666. Furthermore, Cyclin‐dependent kinase 6 (CDK6) was speculated as the downstream target of miR‐1299. In MCF7 and BT474 cells, CDK6 was greatly overexpressed and it was shown that CDK6 contributed a lot to breast cancer progression. Subsequently, it was implied that hsa_circ_0136666 could modulate CDK6 levels positively in vitro. In conclusion, it was revealed that Upregulation of hsa_circ_0136666 promoted breast cancer progression by sponging miR‐1299 and targeting CDK6.