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Effect of cell source and osteoblast differentiation on gene expression profiles of mesenchymal stem cells derived from bone marrow or adipose tissue
Author(s) -
Fideles Simone Ortiz Moura,
Ortiz Adriana Cassia,
Assis Amanda Freire,
Duarte Max Jordan,
Oliveira Fabiola Singaretti,
Passos Geraldo Aleixo,
Beloti Márcio Mateus,
Rosa Adalberto Luiz
Publication year - 2019
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.28463
Subject(s) - mesenchymal stem cell , osteoblast , microbiology and biotechnology , bone marrow , adipose tissue , angiogenesis , cellular differentiation , stem cell transplantation for articular cartilage repair , biology , immunology , adult stem cell , cancer research , gene , endocrinology , genetics , in vitro
Mesenchymal stem cells (MSCs) have been used in therapies for bone tissue healing. The aim of this study was to investigate the effect of cell source and osteoblast differentiation on gene expression profiles of MSCs from bone marrow (BM‐MSCs) or adipose tissue (AT‐MSCs) to contribute for selecting a suitable cell population to be used in cell‐based strategies for bone regeneration. BM‐MSCs and AT‐MSCs were cultured in growth medium to keep MSCs characteristics or in osteogenic medium to induce osteoblast differentiation (BM‐OBs and AT‐OBs). The transcriptomic analysis was performed by microarray covering the entire rat functional genome. It was observed that cells from bone marrow presented higher expression of genes related to osteogenesis, whereas cells from adipose tissue showed a higher expression of genes related to angiogenesis and adipocyte differentiation, irrespective of cell differentiation. By comparing cells from the same source, MSCs from both sources exhibited higher expression of genes involved in angiogenesis, osteoblast differentiation, and bone morphogenesis than osteoblasts. The clustering analysis showed that AT‐OBs exhibited a gene expression profile closer to MSCs from both sources than BM‐OBs, suggesting that BM‐OBs were in a more advanced stage of differentiation. In conclusion, our results suggest that in cell‐based therapies for bone regeneration AT‐MSCs could be considered for angiogenic purposes, whereas BM‐MSCs and osteoblasts differentiated from either source could be better for osteogenic approaches.