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The effects of adipose‐derived stem cells on CD133‐expressing bladder cancer cells
Author(s) -
Maj Malgorzata,
Kokocha Anna,
Bajek Anna,
Drewa Tomasz
Publication year - 2019
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.28436
Subject(s) - paracrine signalling , cancer stem cell , stem cell , cancer research , mesenchymal stem cell , microbiology and biotechnology , cancer cell , pi3k/akt/mtor pathway , adipose tissue , biology , clonogenic assay , chemistry , cancer , cell , signal transduction , medicine , endocrinology , receptor , biochemistry
Mesenchymal stem cells (MSCs) hold great promise as therapeutic agents in regenerative medicine. They are also considered as a preferred cell source for urinary tract reconstruction. However, as MSCs exhibit affinity to tumor microenvironment, possible activation of tumor‐initiating cells remains a major concern in the application of stem cell‐based therapies for patients with a bladder cancer history. To analyze the influence of adipose‐derived stem cells (ASCs) on bladder cancer cells with stem cell‐like properties, we isolated CD133‐positive bladder cancer cells and cultured them in conditioned medium from ASCs (ASC‐CM). Our results showed that parental 5637 and HB‐CLS‐1 cells showed induced clonogenic potential when cultured in ASC‐CM. Soluble mediators secreted by ASCs increased proliferation and viability of unsorted cells as well as CD133+ and CD133− subpopulations. Furthermore, incubation with ASC‐CM modulated activation of intracellular signaling pathways. Soluble mediators secreted by ASCs increased phosphorylation of AKT1/2/3 (1.4‐fold, P < 0.05), ERK1/2 (1.6‐fold, P < 0.02), and p70 S6K (1.4‐fold) in CD133+ cells isolated from 5637 cell line. In turn, decreased phosphorylation of those three proteins involved in PI3K/Akt and MAPK signaling was observed in CD133+ cells isolated from HB‐CLS‐1 cell line. Our results revealed that bladder cancer stem‐like cells are responsive to signals from ASCs. Paracrine factors secreted by locally‐delivered ASCs may, therefore, contribute to the modulation of signaling pathways involved in cancer progression, metastasis, and drug resistance.