Premium
The effects of type I collagen on bone defects and gene expression changes for osteogenesis: In a rat model
Author(s) -
Yiğiter Özgür,
Yorukoglu Ali Cagdas,
Şentürk Nilay,
Dodurga Yavuz,
Demirkan Ahmet Fahir
Publication year - 2019
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.28432
Subject(s) - bone healing , smad , connective tissue , gene expression , type i collagen , wound healing , endocrinology , inflammation , bone morphogenetic protein 2 , medicine , transforming growth factor , chemistry , gene , biology , andrology , pathology , anatomy , immunology , genetics , in vitro
The aim of this study is to investigate the effects of type I collagen on bone defects and on genes specifically for osteogenesis in a rat model. Two millimeter drill hole bone defect was created in the femur of rats. In the experimental group, type I collagen was applied in bone defects whereas in control group defects were left empty. Inflammation, development of connective tissue, osteogenesis, and foreign body reaction parameters evaluated with histologically and genes evaluated by blood samples. In the experimental group, the histopathologically significant change was found in favor of bone healing only at the first week. A significant increase was found in genetic expressions of BMP‐1, 2, 3, 4, 5, 6, 7, TGF‐βRII, Smad‐1, IL‐6, BMPR‐IA, BMPR‐IB, Eng, BMPR‐II, c‐fos, Cdkn1a, Chrd, Gdf‐5, Id‐1, PDGF‐β, IGF‐1, Serpine‐1, and TGF‐βRI at the first hour. At the first, third, and sixth week, no significant increase was found in any of the gene expressions. Type I collagen is found to be effective in favor of bone healing through increased inflammatory cytokines and expression of BMP genes in the early stages of fracture healing.