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RNA‐Seq analysis of differentially expressed genes relevant to mismatch repair in aging hematopoietic stem‐progenitor cells
Author(s) -
Lian Xiaolan,
Dong Yongpin,
Zhao Mingyi,
Liang Yajie,
Jiang Weiwei,
Li Wenfang,
Zhang Lina
Publication year - 2019
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.28417
Subject(s) - msh2 , msh6 , biology , pms2 , dna mismatch repair , gene , mlh1 , stem cell , rna , haematopoiesis , progenitor cell , dna repair , microbiology and biotechnology , genetics , computational biology , cancer research
Abstract We used RNA‐sequencing (RNA‐Seq) technology and an old hematopoietic stem and progenitor cells (HSPCs) model in vitro to analyze differential expressions of mismatch repair (MMR)‐related genes in aged HSPCs, so as to explore the mechanism of DNA MMR injury in hematopoietic stem cells (HSC) aging. In this study, by combining RNA‐seq data and National Center for Biotechnology Information database, we focus on six widely reported MMR genes MSH2, MSH3, MSH6, MLH1, PMS1, PMS2, and five MMR genes with closer ties to HSC aging Pcna, Exo1, Rpa1, Rpa2, and Rpa3 according to the genes functional classification and the related signaling pathway. It is concluded that MMR is closely related to HSC aging. This study provides experimental evidence for future researching MMR in HSC aging.