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miR‐939‐5p decreases the enrichment of RNA polymerase II in the promoter region of CD2AP involved in nephrotic syndrome
Author(s) -
Wang JinYa,
Zhang DaoQi,
Cao Qian,
Qiao XiaoQin,
Zhou GuoPing
Publication year - 2019
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.28413
Subject(s) - promoter , chromatin immunoprecipitation , microbiology and biotechnology , microrna , rna polymerase ii , biology , transcription factor , untranslated region , transcription (linguistics) , reporter gene , hek 293 cells , gene expression , rna , gene , chemistry , genetics , linguistics , philosophy
The expression changes of CD2‐associated protein (CD2AP) can lead to kidney diseases with proteinuria, including nephrotic syndrome (NS). A recent study reported that miRNAs may be important transcriptional regulators. In this study, we found increased expression of miR‐939‐5p and decreased expression of CD2AP in the peripheral blood of patients with NS. However, miR‐939‐5p did not show a regulatory effect on the 3′‐untranslated region of CD2AP. The expression levels of specific protein 1 and adenovirus E2 promoter‐binding factor 1, important transcription regulators in the promoter region of CD2AP, were also not affected by microRNA (miR)‐939‐5p. We confirmed that miR‐939‐5p is in the nucleus by fluorescent in situ hybridization and cytoplasmic separation polymerase chain reaction. The promoter plasmid and miR‐939‐5p were cotransfected into HEK‐293 cells, and the luciferase reporter gene assay was used to analyze the promoter activity. We found that miR‐939‐5p binds to a specific sequence in the CD2AP promoter. miR‐939‐5p was confirmed to reduce the recruitment of RNA polymerase II to the CD2AP promoter region by chromatin immunoprecipitation. These findings improve our understanding of the mechanism of miR‐939‐5p in NS and provide potential molecular therapeutic targets for NS.