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lncRNA FLVCR1‐AS1 silencing inhibits lung cancer cell proliferation, migration, and invasion by inhibiting the activity of the Wnt/β‐catenin signaling pathway
Author(s) -
Lin Heping,
Shangguan Zongxiao,
Zhu Mengchu,
Bao Lianmin,
Zhang Qing,
Pan Shenghua
Publication year - 2019
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.28352
Subject(s) - wnt signaling pathway , gene silencing , catenin , cancer research , microbiology and biotechnology , cell growth , beta catenin , signal transduction , cell , biology , chemistry , genetics , gene
Abstract Long noncoding RNAs have been reported to be essential regulators in several human diseases, including tumorigenesis. A recent report revealed that FLVCR1‐AS1 promotes the progression of hepatocellular carcinoma. However, whether FLVCR1‐AS1 is involved in lung cancer remains unclear. In this study, we found that the expression of FLVCR1‐AS1 was increased in lung cancer tissues according to The Cancer Genome Atlas database. Similarly, FLVCR1‐AS1 was significantly upregulated in lung cancer cell lines. Knockdown of FLVCR1‐AS1 dramatically reduced the cell proliferation, migration, and invasion of SPCA1 and A549. Mechanistically, we found that the expression levels of CTNNB1, SOX4, CCND1, CCND2, c‐MYC, as well as nucleus β‐catenin were decreased in lung cancer cells after FLVCR1‐AS1 silencing. Thus, FLVCR1‐AS1 positively regulates the activation of the Wnt/β‐catenin pathway. Overexpression of CTNNB1 reversed the effect of FLVCR1‐AS1 knockdown on A549 cells. In sum, FLVCR1‐AS1 silencing inhibited the proliferation, migration, and invasion of lung cancer cells by inhibiting the activity of the Wnt/β‐catenin signaling pathway.