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Overexpressed circ_0029426 in glioblastoma forecasts unfavorable prognosis and promotes cell progression by sponging miR‐197
Author(s) -
Zhang Guifang,
Sun Weibo,
Zhu Liangfu,
Feng Yingpu,
Wu Liheng,
Li Tianxiao
Publication year - 2019
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.28313
Subject(s) - glioblastoma , cancer research , oncology , medicine , biology
Accumulating studies indicates that circular RNAs (circRNAs) play an imperative role in modulating cancer progression and metastasis. In the previous study, elevated circ_0029426 was first observed in glioblastoma (GBM) tissues compared with normal tissues by circRNA microarray. Our aim is to study the function and mechanism of circ_0029426 in GBM. Quantitative reverse transcription polymerase chain reaction was used to detect relative circ_0029426 expression in GBM tissue samples and cells. Fisher's exact test was used to evaluate the expression of circ_0029426 and clinical parameters.The Kaplan‐Meier method and Cox regression were analyzed to evaluate the link between circ_0029426 expression and the overall survival of patients with GBM. Loss/gain‐of function experiments were performed to measure GBM cell growth, apoptosis, migration, and invasion. Dual luciferase reporter assays were applied to detect the binding ability between circ_0029426 and miR‐197. As a result, the circ_0029426 expression is tightly correlated with patients’ clinical severity and prognosis. Functionally, circ_0029426 strikingly promoted cell proliferation, migration and invasion, and inhibited cell apoptosis. Mechanistically, miR‐197 was predicted and verified to be sponged by circ_0029426. More importantly, the oncogenic functions of circ_0029426 are partially attributed to its suppression on miR‐197. Collectively, circ_0029426 may be taken as a potential therapeutic target for GBM.