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DDN‐AS1‐miR‐15a/16‐TCF3 feedback loop regulates tumor progression in cervical cancer
Author(s) -
Liu Zhihui,
Wu Meiqin,
Shi Huifeng,
Huang Chong,
Luo Sukun,
Song Xiaojie
Publication year - 2019
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.28307
Subject(s) - gene knockdown , downregulation and upregulation , cancer research , biology , long non coding rna , transcription factor , cell growth , cell culture , gene , genetics
Cervical cancer (CC) is known as one of the most common gynecological tumors. Long noncoding RNAs (lncRNAs) are a group of regulators that have been widely reported in human malignant tumors including CC. On the basis of the data of The Cancer Genome Atlas, lncRNA DDN and PRKAG1 antisense RNA 1 ( DDN‐AS1 ) that is overexpressed in CC tissues predicted poor prognosis for patients with CC. Moreover, quantitative reverse transcription PCR analysis further identified the upregulation of DDN‐AS1 in CC tissues and cell lines. Loss‐of‐function assays revealed that knockdown of DDN‐AS1 suppressed CC progression by efficiently inhibiting cell proliferation, migration, and invasion. Mechanism investigations revealed that DDN‐AS1 was upregulated by its upstream transcription activator transcription factor 3 ( TCF3 ). Moreover, DDN‐AS1 increased the expression of TCF3 by competitively binding miR‐15a and miR‐16 . In conclusion, DDN‐AS1‐miR‐15a/16‐TCF3 feedback loop contributes to cell proliferation, migration, and invasion in CC.