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Electrospun poly‐ l ‐lactic acid/polyvinyl alcohol nanofibers improved insulin‐producing cell differentiation potential of human adipose‐derived mesenchymal stem cells
Author(s) -
Ojaghi Mohammad,
Soleimanifar Fatemeh,
Kazemi Alireza,
Ghollasi Marzieh,
Soleimani Masoud,
Nasoohi Nikoo,
Enderami Seyed Ehsan
Publication year - 2019
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.28274
Subject(s) - mesenchymal stem cell , adipose tissue , tissue engineering , microbiology and biotechnology , chemistry , cd90 , flow cytometry , stem cell , nanofiber , biomedical engineering , biology , materials science , biochemistry , cd34 , medicine , nanotechnology
Combination of adipose‐derived mesenchymal stem cells (ADSCs) and synthetic materials in terms of pancreatic tissue engineering can be considered as a treatment of diabetes. This study aimed to evaluate the differentiation of human ADSCs to pancreatic cells on poly‐ l ‐lactic acid/polyvinyl alcohol (PLLA/PVA) nanofibers as a three‐dimensional (3D) scaffold. Mesenchymal stem cells (MSCs) were characterized for mesenchymal surface markers by flow cytometry. Then ADSCs were seeded on 3D scaffolds and treated with pancreatic differentiation medium. Immunostaining assay showed that ADSCs were very efficiently differentiated into a relatively homogeneous population of insulin‐producing cells. Moreover, real‐time RT‐PCR results revealed that pancreas‐specific markers were highly expressed in 3D scaffolds compared with their expression in tissue culture plates and this difference in expression level was significant. In addition, insulin and C‐peptide secreted in response to varying concentrations of glucose in the 3D scaffold group was significantly higher than that in 2D culture. The results of the present study confirmed that PLLA/PVA scaffold seeded with ADSCs could be a suitable option in pancreatic tissue engineering.