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Elevated expression of circular RNA circ_0008450 predicts dismal prognosis in hepatocellular carcinoma and regulates cell proliferation, apoptosis, and invasion via sponging miR‐548p
Author(s) -
Zhang Juan,
Chang Yuxia,
Xu Lijun,
Qin Lijie
Publication year - 2019
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.28224
Subject(s) - flow cytometry , gene silencing , hepatocellular carcinoma , apoptosis , downregulation and upregulation , cancer research , cell growth , microrna , biology , cell , circular rna , real time polymerase chain reaction , microbiology and biotechnology , gene , genetics
Hepatocellular carcinoma (HCC) is one of the most frequent malignancies and a main cause of global cancer mortality. In the past decade, circular RNAs (circRNAs) have been proved to play key roles in various cancers. Previously, circ_0008450 was identified upregulated in HCC tissues by high‐throughput circRNA sequencing. In this study, quantitative real‐time polymerase chain reaction was used to evaluate the expression level of circ_0008450 in human HCC tumor and corresponding nontumor tissue samples, and the association between circ_0008450 expression and clinicopathologic features of patients with HCC was also analyzed. After that, the functions of circ_0008450 in biological behaviors of HCC cells were determined by cell counting kit‐8, colony formation, flow cytometry, and the transwell assays. The mechanism of circ_0008450 was explored by the bioinformatic analysis and dual‐luciferase reporter assay. The expression of circ_0008450 is upregulated in HCC tissue specimens and cell lines. Patients with a high circ_0008450 expression usually bear a lower 5‐year survival rate. Silencing of circ_0008450 in Huh‐7 cells inhibited cell viability, migration, and invasion, whereas cell apoptosis was increased. Conversely, its overexpression in HepG2 cells leads to absolutely inverse results. In addition, circ_0008450 was proved to be a sponge of miR‐548p. The oncogenic role of circ_0008450 was partially attributed to its suppression on miR‐548p. This study implies a new target for the treatment of HCC.