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Construction and analysis of dysregulated lncRNA‐associated ceRNA network in colorectal cancer
Author(s) -
Zhu Yiping,
Bian Yinzhu,
Zhang Qun,
Hu Jing,
Li Li,
Yang Mi,
Qian Hanqing,
Yu Lixia,
Liu Baorui,
Qian Xiaoping
Publication year - 2019
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.28201
Subject(s) - competing endogenous rna , microrna , biology , long non coding rna , gene , oncogene , messenger rna , colorectal cancer , cancer , gene expression , regulation of gene expression , computational biology , rna , genetics , cancer research , bioinformatics , cell cycle
Colorectal cancer (CRC) is one of the most frequently diagnosed digestive system cancer. The aim of the present study was to investigate the interactions among messenger RNAs (mRNAs), microRNAs (miRNAs), and long noncoding RNAs (lncRNAs) in CRC to reveal the mechanisms of CRC. Differentially expressed genes (DEGs) were identified from public gene expression data sets. One thousand eighty‐one common dysregulated mRNAs in two data sets were identified. Gene function analysis and protein‐protein interaction network analysis indicated that these DEGs might play important roles in CRC. LINC00365 was selected through coding‐ noncoding network analysis and its expression was validated upregulated in 22 paired clinical samples and four CRC cell lines. A competing endogenous RNA network composed of 70 miRNAs, nine mRNAs, and LINC00365 was constructed. Eight of nine mRNAs were validated upregulated in The Cancer Genome Atlas data set. Our results suggested that LINC00365 was an oncogene in CRC and it could regulate the expression of several mRNAs through sponging miRNAs.