Premium
Silencing H19 regulated proliferation, invasion, and autophagy in the placenta by targeting miR‐18a‐5p
Author(s) -
Zhang Lei,
Deng Xinru,
Shi Xian,
Dong Xiaojing
Publication year - 2019
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.28172
Subject(s) - downregulation and upregulation , pi3k/akt/mtor pathway , gene knockdown , gene silencing , autophagy , trophoblast , mapk/erk pathway , cancer research , microrna , biology , competing endogenous rna , protein kinase b , placenta , cell growth , long non coding rna , microbiology and biotechnology , cell culture , signal transduction , fetus , pregnancy , gene , apoptosis , genetics
Fetal growth restriction (FGR) is a serious pregnancy complication associated with increased perinatal mortality and morbidity. It may lead to neurodevelopmental impairment and adulthood onset disorders. Recently, long noncoding RNAs (lncRNAs) were found to be associated with the pathogenesis of FGR. Here we report that the lncRNAH19 is significantly decreased in placentae from pregnancies with FGR. Downregulation of H19 leads to reduced proliferation and invasion of extravillous trophoblast cells. This is identified with reduced trophoblast invasion, which has been discovered in FGR. Autophagy is exaggerated in FGR. Downregulation of H19 promotes autophagy via the PI3K/AKT/mTOR and MAPK/ERK/mTOR pathways of extravillous trophoblast cells in FGR. We also found that the expression level of microRNAs miR‐18a‐5p was negatively correlated with that of H19. H19 can act as an endogenous sponge by directly binding to miR‐18a‐5p, which targets IRF2. The expression of miR‐18a‐5p was upregulated, but IRF2 expression was downregulated after the H19 knockdown. In conclusion, our study revealed that H19 downexpressed could inhibit proliferation and invasion, and promote autophagy by targeting miR‐18a‐5pin HTR8 and JEG3 cells. We propose that aberrant regulation of H19/miR‐18a‐5p‐mediated regulatory pathway may contribute to the molecular mechanism of FGR. We indicated that H19 may be a potential predictive, diagnostic, and therapeutic modality for FGR.